cleaved poly adp ribose polymerase  (Cell Signaling Technology Inc)

Journal: Journal of Clinical and Translational Hepatology

Article Title: Protective Effect of Mesaconate on Autoimmune Hepatitis via Suppression of Inflammatory Response and Oxidative Stress

doi: 10.14218/JCTH.2025.00112

Figure Lengend Snippet: (A) Representative H&E-stained sections of mouse liver tissues with different treatments. (B) Levels of serum ALT and AST in different groups. (C) mRNA levels of Bcl2 and Bax in mouse liver tissue detected by RT-qPCR. (D, E) Protein levels of BCL-2, BAX, C-caspase 3, and C-PARP in mouse liver tissue detected by Western blotting. ns p > 0.05, * p < 0.05, ** p < 0.01, *** p < 0.001, **** p < 0.0001. ConA, concanavalin A; MSA, mesaconate; H&E, hematoxylin and eosin; ALT, alanine aminotransferase; AST, aspartate aminotransferase; BCL2, B-cell lymphoma-2; BAX, Bcl2-associated X protein; C-caspase 3, cleaved-caspase 3; C-PARP, cleaved-poly ADP ribose polymerase; GAPDH, glyceraldehyde 3-phosphate dehydrogenase.

Article Snippet: Antibodies used included: anti-GAPDH (AB0037, Abways), anti-IL-1β (26048-4-AP, Proteintech), anti-apoptotic B-cell lymphoma 2 (26593-1-AP, Proteintech), anti-Bcl2-associated X protein (50599-2-Ig, Proteintech), anti-cleaved-caspase-3 (#9661, Cell Signaling Technology (CST)), anti-cleaved-poly ADP ribose polymerase (#5625, CST), anti-inducible nitric oxide synthase (18985-1-AP, Proteintech), anti-Janus kinase 1 (JAK1) (66466-1-Ig, Proteintech), anti-phosphorylated JAK1 (phos-JAK1) (#74129, CST), anti-JAK2 (#3230, CST), anti-phosphorylated JAK2 (phos-JAK2) (#3771, CST), anti-signal transducer and activator of transcription 1 (STAT1) (10144-2-AP, Proteintech), and anti-phosphorylated STAT1 (phos-STAT1) (28977-1-AP, Proteintech).

Techniques: Staining, Quantitative RT-PCR, Western Blot

Journal: Current Issues in Molecular Biology

Article Title: Reticuline and Coclaurine Exhibit Vitamin D Receptor-Dependent Anticancer and Pro-Apoptotic Activities in the Colorectal Cancer Cell Line HCT116

doi: 10.3390/cimb47100810

Figure Lengend Snippet: Apoptotic status and detection of apoptotic proteins in HCT116-WT and HCT116-VDR/KO cells exposed to VitD 3 , coclaurine, and reticuline. ( A ) Flow cytometry scatter plots of cells treated for 72 h with 0.02% DMSO (negative control), 20 µM of VitD 3 , coclaurine, and reticuline. ( B ) Bar graph of the percentages of HCT116 cell status, determined as viable, early apoptosis, late apoptosis, and necrosis. ( C ) Representative Western blot results showing an increase in the expression levels of cleaved caspase-3, cleaved PARP, and Bax in HCT116-WT cells treated with 20 μM of VitD 3 , coclaurine and reticuline compared with DMSO-treated cells and HCT116-VDR/KO cells. ( D ) Bar graph reporting the quantitative analysis of Western blotting using the ImageJ software. The results are presented as the mean ± SD from three independent experiments. (*), (**), (***) and (****) signify a statistically significant difference ( p < 0.05, p < 0.01, p < 0.001 and p < 0.0001) compared with the DMSO-treated cells.

Article Snippet: Rabbit primary anti-cleaved caspase-3 (#9664), pro-caspase 3 (#9665), cleaved poly (ADP-ribose) polymerase (PARP) (#5625), PARP (#9542), B-cell lymphoma 2 (Bcl-2, #15071S), BCL2-associated X (Bax, #2772S) monoclonal antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: Flow Cytometry, Negative Control, Western Blot, Expressing, Software

Journal: Current Issues in Molecular Biology

Article Title: Reticuline and Coclaurine Exhibit Vitamin D Receptor-Dependent Anticancer and Pro-Apoptotic Activities in the Colorectal Cancer Cell Line HCT116

doi: 10.3390/cimb47100810

Figure Lengend Snippet: This schematic diagram summarizes the investigated anticancer and pro-apoptotic activities of coclaurine and reticuline targeting VDR using CRISPR/Cas9-edited VDR/KO (HCT116-VDR/KO) and HCT116-WT CRC cell lines. HCT116-WT cells exposed to coclaurine and reticuline led to the upregulation of VDR (resulting in the suppression of its target genes SNAIL1 and SNAIL2 ) and the tumor suppressor TP53 , inhibition of the wound-healing process and cell viability. In addition, the cell death mechanisms underlying both alkaloids antiproliferative activities included induction of apoptosis and cell cycle arrest in the S-phase, associated with increased expression of pro-apoptotic proteins (i.e., cleaved PARP, cleaved caspase-3, Bax) and decreased expression of the anti-apoptotic protein (i.e., Bcl-2). However, no biological effects of colcaurine and reticuline were observed in HCT116-VDR/KO cells, demonstrating the crucial role of VDR in both anti-CRC effects of coclaurine and reticuline. Molecular docking study highlighted the strong and weak molecular interactions of reticuline and coclaurine with the active site of VDR, respectively.

Article Snippet: Rabbit primary anti-cleaved caspase-3 (#9664), pro-caspase 3 (#9665), cleaved poly (ADP-ribose) polymerase (PARP) (#5625), PARP (#9542), B-cell lymphoma 2 (Bcl-2, #15071S), BCL2-associated X (Bax, #2772S) monoclonal antibodies were purchased from Cell Signaling Technology (Danvers, MA, USA).

Techniques: CRISPR, Inhibition, Expressing